|
The Imaging Core Facility has three microscopes equipped for
two-photon imaging, two up-right and one inverted microscope.
The intrinsic sectioning capability of two-photon scanning microscopy,
the deep penetration into tissues, and the reduced photo-damage make
this technique particularly useful for live imaging of thick and
scattering samples. Live imaging is therefore the primary application
of these microscopes.
Several additional measurement techniques have been implemented; among
them are fluorescence polarization measurements for the detection of
protein dimer formation by homo-FRET, Fluorescence Correlation
Spectroscopy, and light scattering applications.
These three instruments can be easily modified for particular
applications and the software used to control these microscopes
(written in Matlab) can be customized as well. This provides the
capability to easily tailor imaging sequences, bleach protocols, and
control of peripheral equipment for particular experiments.
The facility is also interested in sub-wavelength resolution
imaging techniques. We have built a PALM microscope which is currently
being tested. This microscope will allow imaging with nanometric range
resolution of fixed sections labeled with photoactivatable fluorescent
proteins.
Last updated:
Copyright © 2007 Lewis-Sigler Institute for Integrative Genomics.
Princeton University. All Rights Reserved.
|
