Overview Equipment Products Protocols Contact Equipment Scheduling Training PUMA db Facility User Responsibilities Site Map

Announcements The Genome Analyzer II is up and running. Samples are being accepted. Please schedule a consultation with Donna and Jessica to go over your experiment and pricing.   All equipment sign up will be done through the google calendar system. To be given permission please follow the instructions on the Equipment Scheduling page.   We will no longer be storing any Agilent or Affy array data on the computers located in the array facility, instead all data will be stored on the array files server.  Linked here.... Affymetrix users Agilent users you will find directions for storing data on this server.  To gain access to this server and to scanning computers you need to fill out the last page on the linked document and return it to Jessica two business days before your next scan.  Facility Overview The Microarray core facility is fully equipped for all aspects of microarray production and data acquisition. Complementary DNA, (cDNA's) and PCR products are amplified and quality checked using the high throughput liquid handling Beckman Coulter Biomek FX, and MJ Research Tetrad thermocyclers. Spotting of PCR products or long Oligomers is accomplished on in-house Arrayers built according to the Joe DeRisi, Pat Brown design and operated by software written by Joe DeRisi. Hybridization probes are checked prior to labeling using the Agilent Bioanalyzer 2100 and the Nanodrop spectrophotometer. Post labeling efficiency is quantified by Nanodrop prior to application of probes to the array. Post-hybridization image acquisition is accomplished using any of two available scanners, an Axon 4200A Professional Scanner equipped with four laser scanning capability, or an Agilent Microarray scanner , two color, equipped with a 48 slide auto-sampler carousel. Data extraction from the images is accomplished using Axon's Genepix software, versions 4.0, 5.1, or 6.0, or Agilent's data feature extraction software v 9.5. The facility also offers access to an Affymetrix Array station consisting of two fluidics stations handling four chips each, a hybridization oven, and a scanner-3000 complete with the "7G" upgrade. GCOS software is available for data analysis. Two computer workstations are available with Acuity 4.0 Software, and one with Pathway Assist Software and one with Genome Studio. The entire lab space is ozone free protecting dye integrity of samples. Personnel are available to contract for any and all aspects above, or to train, assist and troubleshoot do-it-yourselfer's. For inquires or appointments, please email Donna Storton. The Microarray Core Facility is located in the Basement Lab, Room 007. The Entire space is OZONE FREE.The doors are prox accessible, to be granted access CONTACT Donna Storton.